Journal: Clinical Science (London, England : 1979)

Article Title: In vivo inhibition of TDO2 in fibroids results in widespread alteration in the tumor transcriptome

doi: 10.1042/CS20260395

Figure Lengend Snippet: Effects of TDO2 silencing in primary LSMC. Cells were transfected with siTDO2 or control siRNA (siNC) for 96 h, followed by assessment of gene expression by qRT-PCR. Expression levels of TDO2, VDR, MMP11, MMP14, COL11A1, CBX4, LINC02568, LINC01310, LINC02544, LINC02182, and miR-584-5p are shown. Data represent mean ± SEM from four independent experiments ( n = 4). Statistical significance is indicated as * P <0.05 and *** P <0.01.

Article Snippet: For gene silencing experiments, primary LSMCs were transfected with 50 nM of either a non-targeting control siRNA (siNC) or siRNA targeting TDO2 (siTDO2; 5′-CUAUCACUACCUGCGAUCAACUGUG-3′) using PureFection transfection reagent (System Biosciences, Mountain View, CA, U.S.A.), according to the manufacturer’s protocol.

Techniques: Transfection, Control, Gene Expression, Quantitative RT-PCR, Expressing